nap trap on hek293t Search Results


nap trap on hek293t  (ATCC)
99
ATCC nap trap on hek293t
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Nap Trap On Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/293T/bio_rxiv__64898__2026__04__12__718002-170-6-11
Average 99 stars, based on 1 article reviews
nap trap on hek293t - by Bioz Stars, 2026-09
99/100 stars
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hek293t cells  (Proteintech)
96
Proteintech hek293t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek293t Cells, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/HEK+293T+cells+Antibody/bio_rxiv__2025__05__07__652617-221-0-11
Average 96 stars, based on 1 article reviews
hek293t cells - by Bioz Stars, 2026-09
96/100 stars
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hek-293 t  (BioResource International Inc)
90
BioResource International Inc hek-293 t
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293t+cells/pmc04351690-264-2-7
Average 90 stars, based on 1 article reviews
hek-293 t - by Bioz Stars, 2026-09
90/100 stars
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hek–293 t cells  (AddexBio Inc)
90
AddexBio Inc hek–293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek–293 T Cells, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek+293+cells/pmc09291745-42-14-16
Average 90 stars, based on 1 article reviews
hek–293 t cells - by Bioz Stars, 2026-09
90/100 stars
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hek 293 t/17 cells  (JCRB Cell Bank)
90
JCRB Cell Bank hek 293 t/17 cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T/17 Cells, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293+cells/pmc07014756-298-8-18
Average 90 stars, based on 1 article reviews
hek 293 t/17 cells - by Bioz Stars, 2026-09
90/100 stars
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hek 293 t cells  (Promega)
90
Promega hek 293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T Cells, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293+cells/pmc10761673-346-0-8
Average 90 stars, based on 1 article reviews
hek 293 t cells - by Bioz Stars, 2026-09
90/100 stars
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hek-293 t cells  (ScienCell)
90
ScienCell hek-293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293t+cells/pmc09208511-81-0-7
Average 90 stars, based on 1 article reviews
hek-293 t cells - by Bioz Stars, 2026-09
90/100 stars
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hek 293 t cells  (Procell Inc)
86
Procell Inc hek 293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/cells+hek293t/pm41666557-83-10-16
Average 86 stars, based on 1 article reviews
hek 293 t cells - by Bioz Stars, 2026-09
86/100 stars
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hek 293 t cells  (Ribobio co)
90
Ribobio co hek 293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T Cells, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293t+cells/10__1016_slash_j__smallrumres__2019__06__004-33-0-7
Average 90 stars, based on 1 article reviews
hek 293 t cells - by Bioz Stars, 2026-09
90/100 stars
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lipod293  (SignaGen)
90
SignaGen lipod293
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Lipod293, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/lipod293/pmc08726646-139-3-30
Average 90 stars, based on 1 article reviews
lipod293 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
hek 293 t cells  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare hek 293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T Cells, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293t+cells/pmc05527104-295-0-11
Average 90 stars, based on 1 article reviews
hek 293 t cells - by Bioz Stars, 2026-09
90/100 stars
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hek-293 t cells  (iCell Bioscience Inc)
90
iCell Bioscience Inc hek-293 t cells
Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in <t>HEK293T,</t> H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).
Hek 293 T Cells, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nap+trap+on+hek293t/hek293t+cells/pmc11427634-43-0-6
Average 90 stars, based on 1 article reviews
hek-293 t cells - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in HEK293T, H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).

Journal: bioRxiv

Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability

doi: 10.64898/2026.04.12.718002

Figure Lengend Snippet: Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in HEK293T, H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).

Article Snippet: Cultured cell lines We have used NaP-TRAP on HEK293T (RRID: CVCL_0063, ATCC Cat. No. CRL-3216) but successfully performed it in various other cell lines including MCF7 (RRID: CVCL_0031, ATCC Cat. No. HTB-22) and H9 (RRID:CVCL_1240, ATCC Cat. No. HTB-176) .

Techniques: In Vivo, Microinjection, In Vitro, Transfection, Purification, Sequencing, Injection, Control

Expected results from NaP-TRAP MPRA analysis pipeline. ( A ) Example histogram of per-insert read counts for one Input replicate with 7,839,828 reads. ( B ) Representative replicate-to-replicate correlations of NaP-TRAP translation efficiency (TE; Pulldown/Input) at 2 hpf and 6 hpf ( R values are Pearson correlations). ( C ) Hierarchical clustering heatmap of Pearson correlations showing TE similarity across replicates and conditions (zebrafish 2 hpf, zebrafish 6 hpf, and HEK293T). ( D ) Distribution of NaP-TRAP TE values at 2 hpf, with the top and bottom 10% of reporters highlighted as activated (blue) and repressed (orange), respectively. ( E ) Example k-mer enrichment analysis for activated and repressed reporter sets at 2 hpf. ( F ) Scatter plot comparing reporter NaP-TRAP TE at 2 versus 6 hpf, highlighting four reporter sets: reporters activated (blue) and repressed (orange) at both stages, reporters with higher TE at 2 hpf than at 6 hpf (2 hpf activated; green), and reporters with high TE at 6 hpf than at 2 hpf (6 hpf activated; pink). ( G ) k-mer enrichment analyses for each of the four reporter sets defined in (F): 2 hpf activated (green), 6 hpf activated (pink), globally repressed (orange) and globally activated (blue).

Journal: bioRxiv

Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability

doi: 10.64898/2026.04.12.718002

Figure Lengend Snippet: Expected results from NaP-TRAP MPRA analysis pipeline. ( A ) Example histogram of per-insert read counts for one Input replicate with 7,839,828 reads. ( B ) Representative replicate-to-replicate correlations of NaP-TRAP translation efficiency (TE; Pulldown/Input) at 2 hpf and 6 hpf ( R values are Pearson correlations). ( C ) Hierarchical clustering heatmap of Pearson correlations showing TE similarity across replicates and conditions (zebrafish 2 hpf, zebrafish 6 hpf, and HEK293T). ( D ) Distribution of NaP-TRAP TE values at 2 hpf, with the top and bottom 10% of reporters highlighted as activated (blue) and repressed (orange), respectively. ( E ) Example k-mer enrichment analysis for activated and repressed reporter sets at 2 hpf. ( F ) Scatter plot comparing reporter NaP-TRAP TE at 2 versus 6 hpf, highlighting four reporter sets: reporters activated (blue) and repressed (orange) at both stages, reporters with higher TE at 2 hpf than at 6 hpf (2 hpf activated; green), and reporters with high TE at 6 hpf than at 2 hpf (6 hpf activated; pink). ( G ) k-mer enrichment analyses for each of the four reporter sets defined in (F): 2 hpf activated (green), 6 hpf activated (pink), globally repressed (orange) and globally activated (blue).

Article Snippet: Cultured cell lines We have used NaP-TRAP on HEK293T (RRID: CVCL_0063, ATCC Cat. No. CRL-3216) but successfully performed it in various other cell lines including MCF7 (RRID: CVCL_0031, ATCC Cat. No. HTB-22) and H9 (RRID:CVCL_1240, ATCC Cat. No. HTB-176) .

Techniques: